Fluorescence results from a process that occurs under certain condition in molecules known as fluophores, or fluorescent dyes.
First, the fluophore absorbs the UV light coming from the transilluminator. Then, the fluophore is excited to a higher energy state and emits fluorescent light. The emission wavelength is always higher than the excitation wavelength (UV light).
The aim of the gel documentation system is to separate the emitted light from the excitation light in order to obtain a gel image.
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